|
|
|
| Registros recuperados: 28 | |
|
|
| JING, Zhang; MAGONA, Joseph W; SAKURAI, Tatsuya; THEKISOE, Oriel M. M; OTIM, Charles P; SUGIMOTO, Chihiro; INOUE, Noboru; 井上, 昇. |
| Prevalence of bovine trypanosomosis was determined from a total of 203 blood samples collected from Butaleja district, eastern Uganda. All samples were examined by microhematocrit centrifuge test (MHC), PCR and ELISA. ELISA was performed in accordance with the OIE standard procedures using Trypanosoma brucei gambiense procyclic form crude antigens. PCR were utilized to identify the species and the subspecies of trypanosome. The overall prevalence of bovine African trypanosomosis was 8.9% by MHC, and 45.3% by the ELISA. Since substantial number (12 out of 18) of MHC positive samples were negative in the PCR tests, we could not conclude the most epidemic trypanosome species in the studied area. Nevertheless, the PCR results suggests that the most prevalent... |
|
Palavras-chave: Bovine; Prevalence; Trypanosome; Uganda; Zoonotic parasite. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2690 |
| |
|
|
| Kuboki, Noritaka; Yokoyama, Naoaki; Namangala, B; Okamura, Masashi; Inoue, Noboru; Takagi, Hideaki; Nakayama, Tomoko; Nishikawa, Yoshifumi; Ikehara, Yuzuru; Kojima, Naoya; 横山, 直明; 井上, 昇; 西川, 義文. |
|
Palavras-chave: Adjuvant; African trypanosome; Immunization; Oligomannose-coated liposome(OML). |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2222 |
| |
|
|
| NGUYEN, Thu-Thuy; MOTSIRI, Mono Sophie; TAIOE, Moeti Oriel; MTSHALI, Moses Sibusiso; GOTO, Yasuyuki; KAWAZU, Shin-Ichiro; Molifi THEKISOE, Oriel Matlhahane; INOUE, Noboru; 河津, 信一郎; 井上, 昇. |
| A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8–46.3% and 0–9.1%, respectively. Out of 9 sheep serum samples, 2–4 sera (22.2–44.4%) were positive. The detection performance of crude and recombinant... |
|
Palavras-chave: Animal African trypanosomosis; ELISA; Immunochromatographic test; Serodiagnosis; South Africa. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4034 |
| |
|
|
| MOSSAAD, Ehab; ASADA, Masahito; NAKATANI, Daichi; INOUE, Noboru; YOKOYAMA1), Naoaki; KANEKO, Osamu; KAWAZU, Shin-ichiro; 井上, 昇; 横山, 直明; 河津, 信一郎. |
| Bovine babesiosis is a livestock disease known to cause economic losses in endemic areas. The apicomplexan parasite Babesia bovis is able to invade and destroy the host’s erythrocytes leading to the serious pathologies of the disease, such as anemia and hemoglobinuria. Understanding the egress mechanisms of this parasite is therefore a key step to develop new therapeutic strategies. In this study, the possible involvement of Ca2+ in the egress of B. bovis merozoites from infected erythrocytes was investigated. Egress was artificially induced in vitro using calcium ionophore A23187 and thapsigargin to increase Ca2+ concentration in the cytosol of the parasite cells. The increased intracellular Ca2+ concentration following these treatments was confirmed... |
|
Palavras-chave: Babesia bovis; Calcium ionophore; Calcium signalling; Egress. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4071 |
| |
|
|
| Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志. |
|
Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624 |
| |
|
| |
|
| |
|
|
| Hakimi, Hassan; Nguyen, Thu-Thuy; Suganuma, Keisuke; Masuda-Suganuma, Hirono; Ma. M. Angeles, Jose; Inoue, Noboru; Kawazu, Shin-ichiro; 井上, 昇; 河津, 信一郎. |
| Prompt and accurate diagnosis of malarial patients is a crucial factor in controlling the morbidity and mortality of the disease. Effective treatment decisions require a correct diagnosis among mixed-species malarial patients. Differential diagnosis is particularly important in cases of Plasmodium vivax, a species that shares endemicity with P. falciparum in most endemic areas. Moreover, it is difficult to identify P. knowlesi on the basis of morphology alone, and rapid diagnostic tests are still not available for this malaria species. Therefore, the development of diagnostic tests applicable to the field is urgently needed. 1-Cys peroxiredoxin (1-Cys-Prx) in P. falciparum is abundantly expressed in the mature asexual stages, making it a promising... |
|
Palavras-chave: Malaria diagnosis; Monoclonal antibody; Peroxiredoxin; Plasmodium knowlesi; P. vivax. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3859 |
| |
|
|
| Xuan, Xuenan; Igarashi, Ikuo; Avarzed, A.; Ikadai, Hiromi; Inoue, Noboru; Nagasawa, Hideyuki; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男; 井上, 昇. |
| A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi, and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction（PCR）method．The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001％. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting... |
|
Palavras-chave: Babesia caballi; PCR; IFAT. |
| Ano: 1998 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/281 |
| |
|
| |
|
| |
|
|
| SUGANUMA, Keisuke; ALLAMANDA, Puttik; HAKIMI, Hassan; ZHOU, Mo; ANGELES, Jose Ma.; KAWAZU, Shin-ichiro; INOUE, Noboru; 河津, 信一郎; 井上, 昇. |
| Animal African trypanosomosis (AAT), caused by Trypanosoma congolense, is widespread throughout sub-Saharan Africa. There are significant concerns related to the current drugs available for the treatment of AAT due to their limited effectiveness across species and their adverse effects. Moreover, drug resistant trypanosomes have recently been reported in the field. High throughput screening (HTS) of large chemical compound library collections is a promising approach for identifying novel drug candidates. While HTS for Trypanozoon trypanosomes, T. brucei sspp. and T. evansi is well established, no assays have been developed for T. congolense. In the present study, the authors developed an ATP-based luciferase viability assay for T. congolense in a 96-well... |
|
Palavras-chave: Animal African trypanosomosis; Drug screening; Luciferase assay; Trypanosoma congolense. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3984 |
| |
|
|
| Tuvshintulga, B.; Battsetseg, B.; Battur, B.; Myagmarsuren, P.; Narantsatsral, S.; Sivakumar, T.; Takemae, H.; Igarashi, I.; Inoue, N.; Yokoyama, N.; 五十嵐, 郁男; 井上, 昇; 横山, 直明. |
| Babesia venatorum, formerly known as Babesia sp. EU1, is a zoonotic hemoprotozoan parasites that commonly infects deer. In the present study, we investigated B. venatorum infection in Ixodes persulcatus, an important tick vector capable of transmitting several tick-borne pathogens that cause babesiosis, encephalitis, tularemia, and Lyme diseases. DNA samples extracted from questing I. persulcatus ticks (n=63) that had been collected in Selenge province of Mongolia in 2012 and 2013 were screened for B. venatorum using a nested PCR assay. The findings showed that two of 63 DNA samples were positive for B. venatorum. The 18S rRNA sequences amplified from B. venatorum-positive DNA samples shared high identity scores (96.1–99.9%) with known B. venatorum... |
|
Palavras-chave: Babesia venatorum; 18S rRNA; Ixodes persulcatus; Mongolia. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4243 |
| |
|
|
| ZHOU, Mo; SUGANUMA, Keisuke; RUTTAYAPORN, Ngasaman; NGUYEN, Thu-Thuy; YAMASAKI, Shino; IGARASHI, Ikuo; KAWAZU, Shin-ichiro; SUZUKI, Yasuhiko; INOUE, Noboru; 五十嵐, 郁男; 井上, 昇. |
| Trypanosoma congolense is a major livestock pathogen in Africa, causing large economic losses with serious effects on animal health. Reliable serodiagnostic tests are therefore urgently needed to control T. congolense infection. In this study, we have identified one T. congolense protein as a new candidate serodiagnostic antigen. The 46.4 kDa protein (TcP46, Gene ID: TcIL3000.0.25950) is expressed 5.36 times higher in metacyclic forms than epimastigote forms. The complete nucleotide sequences of TcP46 contained an open reading frame of 1,218 bp. Southern blot analysis indicated that at least two copies of the TcP46 gene were tandemly-arranged in the T. congolense genome. The recombinant TcP46 (rTcP46) was expressed in Escherichia coli as a glutathione... |
|
Palavras-chave: Diagnosis; ELISA; Nagana; TcP46; Trypanosoma. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3983 |
| |
|
|
| Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Suzuki, R.; Kato, Mihoko; Bray, R. L.; Lim, C.; Zayatiin, Batsukh; Kamada, Takenori; Inoue, Noboru; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 玄, 学南. |
|
Palavras-chave: SAG-1; Oral wash and two-step polymerase chain reaction. |
| Ano: 1999 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/311 |
| |
|
|
| SIVAKUMAR, Thillaiampalam; LAN, Dinh Thi Bich; LONG, Phung Thang; YOSHINARI, Takeshi; TATTIYAPONG, Muncharee; GUSWANTO, Azirwan; OKUBO, Kazuhiro; IGARASHI, Ikuo; INOUE, Noboru; XUAN, Xuenan; YOKOYAMA, Naoaki; 井上, 昇; 玄, 学南; 横山, 直明. |
| Hemoprotozoan infections often cause serious production losses in livestock. In the present study, we conducted a PCR-based survey of Babesia bovis, Babesia bigemina, Theileria annulata, Theileria orientalis, Trypanosoma evansi and Trypanosoma theileri, using 423 DNA samples extracted from blood samples of cattle (n=202), water buffaloes (n=43), sheep (n=51) and goats (n=127) bred in the Hue and Hanoi provinces of Vietnam. With the exception of T. annulata and T. evansi, all other parasite species (B. bovis, B. bigemina, T. orientalis and T. theileri) were detected in the cattle populations with B. bovis being the most common among them. Additionally, four water buffaloes and a single goat were infected with B. bovis and B. bigemina, respectively. The Hue... |
|
Palavras-chave: Epidemiology; Hemoprotozoa; Livestock; PCR; Vietnam. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4015 |
| |
|
| |
|
|
| Namangala, Boniface; Inoue, Noboru; Sugimoto, Chihiro; 井上, 昇. |
| Transforming growth factor beta-1 (TGF-β1) is a pleiotropic cytokine with both pro- and antiinflammatory properties, depending on its environment and concentration. The present study evaluated the effects of orally-delivered TGF-β1 on mice parenterally-infected with various protozoan parasites. We report that while orally-administered TGF-β1 seems to confer partial protection against murine chronic babesiosis and acute trypanosomosis, no beneficial clinical effects were observed against acute babesiosis, malaria or toxoplasmosis. Taken together, these preliminary data suggest that the systemic effects conferred by exogenous TGF-β1 could be parasite speciesspecific. The variations in different parasitic infections could be due to (i) intrinsic differences... |
|
Palavras-chave: Low-dose; Mice; Orally-delivered TGF-β1; Protection; Protozoan parasites. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2716 |
| |
|
|
| Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Inoue, Noboru; Igarashi, Ikuo; Nagasawa, Hideyuki; Horiuchi, Motohiro; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 五十嵐, 郁男; 長澤, 秀行. |
|
Palavras-chave: Toxoplasma gondii; SAG-1 (p30); Transgenic mice. |
| Ano: 1996 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/254 |
| |
|
|
| THEKISOE, Oriel M. M; BAZIE, Raoul S. B; CORONEL-SERVIAN, Andrea M; SUGIMOTO, Chihiro; KAWAZU, Shin-ichiro; INOUE, Noboru; 井上, 昇. |
| This study evaluated the stability of LAMP reagents when stored at 25C and 37C, and also assessed its detection efficiency on different DNA template preparations. Accordingly, LAMP using reagents stored at 25C and 37C amplified DNA of in vitro cultured T. b. brucei (GUTat 3.1) from day 1 to day 15 of reagent storage. There were no significant differences (P>0.05) in detection sensitivity of LAMP among the reagents stored at 25C, 37C and –20C (recommended storage temperature). LAMP using the reagents stored at above-mentioned temperatures amplified serially diluted DNAs (genomic DNA extracted by phenol-chloroform method, FTA card and hemolysed blood) of T. b. gambiense (IL2343) with high sensitivity. Reactions were conducted on the reagents stored... |
|
Palavras-chave: Diagnosis; DNA template; LAMP; Parasitic disease; Trypanosoma. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2691 |
| |
| Registros recuperados: 28 | |
|
|
|
